包含所有DAB染色所需要的试剂。工作液与HRP检测系统反应,比如SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125),呈棕色显色。 储存: 4ºC,避免受光。12个月。准备DAB工作液,加1滴(30μl) SignalStain® DAB Chromogen Concentrate到1毫升S SignalStain® DAB Diluent并混合使用。工作液可在4ºC储存14天或在室温下(大约25ºC) 储存5天。变色对染色的质量没有影响。用吸管加30μl SignalStain® DAB Chromogen Concentrate到1毫升SignalStain® DAB Diluent中。洗片并在室温下与工作液孵育到染色。1 - 10分钟通常可获得足够的染色强度。在水中洗片6分钟。如果需要可进行复染。 Contains all of the necessary reagents to prepare a working solution of diaminobenzidine (DAB) for staining tissue sections. The DAB working solution reacts with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125), yielding a brown reaction product.
包含所有DAB染色所需要的试剂。工作液与HRP检测系统反应,比如SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125),呈棕色显色。 储存: 4ºC,避免受光。12个月。准备DAB工作液,加1滴(30μl) SignalStain® DAB Chromogen Concentrate到1毫升S SignalStain® DAB Diluent并混合使用。工作液可在4ºC储存14天或在室温下(大约25ºC) 储存5天。变色对染色的质量没有影响。用吸管加30μl SignalStain® DAB Chromogen Concentrate到1毫升SignalStain® DAB Diluent中。洗片并在室温下与工作液孵育到染色。1 - 10分钟通常可获得足够的染色强度。在水中洗片5分钟。如果需要可进行复染。 Contains all of the necessary reagents to prepare a working solution of diaminobenzidine (DAB) for staining tissue sections. The DAB working solution reacts with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125), yielding a brown reaction product.
IHC配套试剂盒;Immunohistochemistry Application Solutions Kit (Rabbit)旨在方便地提供石蜡包埋组织样本或细胞颗粒(IHC-P)的免疫组化染色试剂。The Immunohistochemistry Application Solutions Kit (IHC-P) is designed to conveniently provide supporting reagents needed for immunohistochemistry staining in paraffin-embedded tissue samples or cell pellets (IHC-P). The reagents in this kit are thoroughly validated using our IHC-recommended rabbit polyclonal and monoclonal antibodies and will perform optimally with the CST immunohistochemistry staining protocol, ensuring accurate and reproducible results. This kit includes sufficient reagents for 120 slides based on a 100 µl assay volume. All reagents in this kit are available individually.
IMPORTANT: Please refer to the primary antibody data sheet to determine if the antibody is approved for use on paraffin-embedded samples (IHC-P) and for information regarding the appropriate antibody dilution, diluent, and antigen unmasking procedure.
System includes 1.0 ml normal mouse blocking serum, 250 µg mouse monoclonal anti-rabbit IgG-Biotin secondary, avidin and biotinylated horseradish peroxidase (ABC reagents), peroxidase substrate, DAB chromogen and DAB dilution buffer.Tissue sections are first incubated with primary antibody. Biotinylated secondary antibody is added, followed by the addition of preformed ABC reagent. The target protein is visualized by incubation in peroxidase substrate.Number of slides: 200
System includes 1.0 ml normal mouse blocking serum, 250 µg mouse monoclonal anti-goat IgG-Biotin secondary, avidin and biotinylated horseradish peroxidase (ABC reagents), peroxidase substrate, DAB chromogen and DAB dilution buffer.Tissue sections are first incubated with primary antibody. Biotinylated secondary antibody is added, followed by the addition of preformed ABC reagent. The target protein is visualized by incubation in peroxidase substrate.Number of slides: 200
System includes peroxidase blocking solution, normal mouse blocking serum, mouse monoclonal anti-rabbit IgG-Biotin secondary and Avidin D-HRP reagent.Each of the above reagents is pre-diluted and ready to use for immunohistochemical staining of tissue sections.Also included are peroxidase substrate, DAB chromogen and DAB dilution buffer.Number of slides: 150
System includes peroxidase blocking solution, normal mouse blocking serum, mouse monoclonal anti-goat IgG-Biotin secondary and Avidin D-HRP reagent.Each of the above reagents is pre-diluted and ready to use for immunohistochemical staining of tissue sections.Also included are peroxidase substrate, DAB chromogen and DAB dilution buffer.Number of slides: 150
0.5 ml Reagent A (avidin)0.5 ml Reagent B (biotinylated-HRP (horseradish peroxidase))Sufficient for 200 slidesRecommended when using the Avidin-Biotin Complex (ABC) staining method for immunoperoxidase staining of tissues (IHC) or cells (ICC)ImmunoCruz® ABC Kit provides increased sensitivity with decreased backgroundRecommended for use with conventional species-specific biotinylated secondary antibodiesNote: this kit is not species-specific. Secondary antibody is not included.For complementary reagents to complete your ABC staining protocol, see also: UltraCruz® Blocking Reagent (sc-516214), Hydrogen Peroxide (sc-203336) and DAB, 50X (sc-24982)Store at 4° C