包含所有DAB染色所需要的试剂。工作液与HRP检测系统反应,比如SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125),呈棕色显色。 储存: 4ºC,避免受光。12个月。准备DAB工作液,加1滴(30μl) SignalStain® DAB Chromogen Concentrate到1毫升S SignalStain® DAB Diluent并混合使用。工作液可在4ºC储存14天或在室温下(大约25ºC) 储存5天。变色对染色的质量没有影响。用吸管加30μl SignalStain® DAB Chromogen Concentrate到1毫升SignalStain® DAB Diluent中。洗片并在室温下与工作液孵育到染色。1 - 10分钟通常可获得足够的染色强度。在水中洗片6分钟。如果需要可进行复染。 Contains all of the necessary reagents to prepare a working solution of diaminobenzidine (DAB) for staining tissue sections. The DAB working solution reacts with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125), yielding a brown reaction product.

TMB Substrate 在 ELISA 检测中可即用。在 ELISA 孔中，底物和固定辣根过氧化物酶 (HRP) 标记二抗的反应会产生蓝色溶液。颜色强度和显色时间会根据检测敏感度和条件有所变化。达到所需颜色强度后，添加酸性 STOP solution 使反应终止，从而使溶液颜色从蓝色变为黄色。终止后，结果会保持稳定达 1 小时，应立即在微孔读板机的 450 nm 处对平板进行分析。TMB 对光敏感，因此装在琥珀色瓶中，以保护溶液免受阳光直射。建议的保存温度为 4ºC。

包含所有DAB染色所需要的试剂。工作液与HRP检测系统反应,比如SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125),呈棕色显色。 储存: 4ºC,避免受光。12个月。准备DAB工作液,加1滴(30μl) SignalStain® DAB Chromogen Concentrate到1毫升S SignalStain® DAB Diluent并混合使用。工作液可在4ºC储存14天或在室温下(大约25ºC) 储存5天。变色对染色的质量没有影响。用吸管加30μl SignalStain® DAB Chromogen Concentrate到1毫升SignalStain® DAB Diluent中。洗片并在室温下与工作液孵育到染色。1 - 10分钟通常可获得足够的染色强度。在水中洗片5分钟。如果需要可进行复染。 Contains all of the necessary reagents to prepare a working solution of diaminobenzidine (DAB) for staining tissue sections. The DAB working solution reacts with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125), yielding a brown reaction product.

STOP Solution 是一种在 ELISA 应用中用于终止过氧物酶/TMB 反应的专有溶液。TMB substrate 会与固定辣根过氧化物酶 (HRP) 标记二抗发生反应，从而产生蓝色溶液。颜色强度指示分析物的水平。获得所需强度后，加入 STOP Solution 来终止反应。加入 STOP Solution 时，颜色从蓝色变为黄色。立即读取在 450 nm 处的吸光度，反应会保持稳定一小时。

IHC配套试剂盒；Immunohistochemistry Application Solutions Kit (Rabbit)旨在方便地提供石蜡包埋组织样本或细胞颗粒(IHC-P)的免疫组化染色试剂。The Immunohistochemistry Application Solutions Kit (IHC-P) is designed to conveniently provide supporting reagents needed for immunohistochemistry staining in paraffin-embedded tissue samples or cell pellets (IHC-P). The reagents in this kit are thoroughly validated using our IHC-recommended rabbit polyclonal and monoclonal antibodies and will perform optimally with the CST immunohistochemistry staining protocol, ensuring accurate and reproducible results. This kit includes sufficient reagents for 120 slides based on a 100 µl assay volume. All reagents in this kit are available individually. IMPORTANT: Please refer to the primary antibody data sheet to determine if the antibody is approved for use on paraffin-embedded samples (IHC-P) and for information regarding the appropriate antibody dilution, diluent, and antigen unmasking procedure.

System includes 1.0 ml normal mouse blocking serum, 250 µg mouse monoclonal anti-rabbit IgG-Biotin secondary, avidin and biotinylated horseradish peroxidase (ABC reagents), peroxidase substrate, DAB chromogen and DAB dilution buffer.Tissue sections are first incubated with primary antibody. Biotinylated secondary antibody is added, followed by the addition of preformed ABC reagent. The target protein is visualized by incubation in peroxidase substrate.Number of slides: 200

System includes 1.0 ml normal mouse blocking serum, 250 µg mouse monoclonal anti-goat IgG-Biotin secondary, avidin and biotinylated horseradish peroxidase (ABC reagents), peroxidase substrate, DAB chromogen and DAB dilution buffer.Tissue sections are first incubated with primary antibody. Biotinylated secondary antibody is added, followed by the addition of preformed ABC reagent. The target protein is visualized by incubation in peroxidase substrate.Number of slides: 200

System includes peroxidase blocking solution, normal mouse blocking serum, mouse monoclonal anti-rabbit IgG-Biotin secondary and Avidin D-HRP reagent.Each of the above reagents is pre-diluted and ready to use for immunohistochemical staining of tissue sections.Also included are peroxidase substrate, DAB chromogen and DAB dilution buffer.Number of slides: 150

System includes peroxidase blocking solution, normal mouse blocking serum, mouse monoclonal anti-goat IgG-Biotin secondary and Avidin D-HRP reagent.Each of the above reagents is pre-diluted and ready to use for immunohistochemical staining of tissue sections.Also included are peroxidase substrate, DAB chromogen and DAB dilution buffer.Number of slides: 150

终止液在ELISA中用于终止过氧化物酶/TMB的反应。底物和固定的辣根过氧化物酶（HRP）结合的二抗之间的反应生成蓝色的溶液。颜色的深度与分析物的含量成比例。达到所需要的颜色深度后，加入酸性终止液可以使反应溶液的颜色右蓝色变为黄色。终止反应1小时内，结果可以保持稳定。板子应该在尽快用读板机于450nm读取并分析结果。

TMB Substrate 在 ELISA 检测中可即用。在 ELISA 孔中，底物和固定辣根过氧化物酶 (HRP) 标记二抗的反应会产生蓝色溶液。颜色强度和显色时间会根据检测敏感度和条件有所变化。达到所需颜色强度后，添加酸性 STOP solution 使反应终止，从而使溶液颜色从蓝色变为黄色。终止后，结果会保持稳定达 1 小时，应立即在微孔读板机的 450 nm 处对平板进行分析。TMB 对光敏感，因此装在琥珀色瓶中，以保护溶液免受阳光直射。建议的保存温度为 4ºC。

TMB Substrate 在 ELISA 检测中可即用。在 ELISA 孔中，底物和固定辣根过氧化物酶 (HRP) 标记二抗的反应会产生蓝色溶液。颜色强度和显色时间会根据检测敏感度和条件有所变化。达到所需颜色强度后，添加酸性 STOP solution 使反应终止，从而使溶液颜色从蓝色变为黄色。终止后，结果会保持稳定达 1 小时，应立即在微孔读板机的 450 nm 处对平板进行分析。TMB 对光敏感，因此装在琥珀色瓶中，以保护溶液免受阳光直射。建议的保存温度为 4ºC。

ELISA应用中，作为HRP的底物

终止液在ELISA中用于终止过氧化物酶/TMB的反应。底物和固定的辣根过氧化物酶（HRP）结合的二抗之间的反应生成蓝色的溶液。颜色的深度与分析物的含量成比例。达到所需要的颜色深度后，加入酸性终止液可以使反应溶液的颜色右蓝色变为黄色。终止反应1小时内，结果可以保持稳定。板子应该在尽快用读板机于450nm读取并分析结果。

Available as 50 ml

0.5 ml Reagent A (avidin)0.5 ml Reagent B (biotinylated-HRP (horseradish peroxidase))Sufficient for 200 slidesRecommended when using the Avidin-Biotin Complex (ABC) staining method for immunoperoxidase staining of tissues (IHC) or cells (ICC)ImmunoCruz® ABC Kit provides increased sensitivity with decreased backgroundRecommended for use with conventional species-specific biotinylated secondary antibodiesNote: this kit is not species-specific. Secondary antibody is not included.For complementary reagents to complete your ABC staining protocol, see also: UltraCruz® Blocking Reagent (sc-516214), Hydrogen Peroxide (sc-203336) and DAB, 50X (sc-24982)Store at 4° C